Research Synopsis:
Molecular imaging, defined as the in vivo characterization of biological processes at the cellular and molecular level, is an attempt to image the molecular make-up of the macrofeatures currently visualized using “classical” diagnostic imaging techniques. Classically, detection of malignant tumor cells in a background of normal or hyperplastic benign tissue is often based on differences in physical properties between tissues, which are frequently minimal, resulting in low contrast resolution.
My laboratory concentrates on research related to the development and application of Molecular Imaging technologies. Specifically we are interested in designing novel imaging tools to image individual and multiple molecular markers of disease. We believe that imaging tissues based on the underlying molecular rather than macromolecular differences will result in a substantial increase in the sensitivity and specificity of medical imaging. Some areas of active research include defining “molecular signatures” of disease, developing paradigms to image multiple markers of disease simultaneously, developing methods to image intracellular disease markers in breast cancer, and developing molecular imaging technologies that allow for intraoperative assessment of disease progression.
Selected Articles:
Weissleder, R., Moore, A.,Ph.D., Mahmood-Bhorade, U., Benveniste, H. Chiocca, E.A., Basilion, J.P. High resolution in vivo imaging of transgene expression, Nature Medicine, 6:351-355, 2000.
Hogeman, D., Josephson, J., Weissleder, R., and Basilion, J.P., Improvement of MRI Probes to Allow Efficient Detection of Gene Expression, Bioconjugate Chemistry, 2000, 6(11): 941-946.
Moore, A., Joshpson, L., Bhorade, R., Basilion, J.P., and Weissleder, R. Human Transferrin Receptor Gene as a Marker Gene for MR Imaging. Radiology, 2001, 221(1): 244-50.
Ichikawa , T., Hogemann, D., Saeki, Y., Tyminski, E., Terada, K., Weissleder, R., Chiocca, E.A., and Basilion, J.P. MR Imaging of Transgene Expression: Correlation to Therapeutic Gene Expression. Neoplasia, 2002, vol 4, No. 6, pp. 523-530.
Abe, T., Terada, K., Wakimoto, H., Bookstein, R., Basilion, J.P. , and Chiocca, E.A. PTEN decreases in vivo vascularization of experimental gliomas in spite of pro-angiogenic stimuli. Cancer Research, 2003 May 1;63(9):2300-5.
Savellano, D.H., Bos, E., Blondet, C., Sato, F., Abe, T., Josephson, L., Weissleder, R., Gaudet, J., Sgroi, D., Peters, P.J., and Basilion, J.P. The Transferrin Receptor: A Potential Molecular Imaging Marker for Human Cancer. Neoplasia, 2003 5(6): 495-506.
Tsourkas, A., Newton, G., Perez, J., Basilion, J.P., and Weissleder, R. (2005). Detection of Peroxidase-Mediated Dityrosine Formation with Enhanced Yellow Fluorescent Protein. Anal Chem. 2005 May 1;77(9):2862-7.
Sato, F., Fukuhara, H., and Basilion, J.P. (2005) Effects of Hormone Deprivation and 2-Methoxyestradiol Combination Therapy on Hormone Dependent Prostate Cancer In Vivo. Neoplasia Sep;7(9):838-46.
Reviews:
Basilion, J.P. (editor), Breast imaging: New technologies for high resolution non-invasive imaging., Thematic review in: Breast Cancer Research, 8:14-16, 2001.
Hogemann D. and Basilion, J.P. “Seeing Inside the Body”: MR Imaging of gene expression, Thematic review in: European Journal of Nuclear Medicine, Mar;29(3):400-8, 2002.
Basilion, J.P. The promise and reality of Molecular Imaging for drug development . Invited Commentary for Preclinica, September 2004.
Ichikawa T, Terada K, Tamiya T, Basilion JP, Chiocca EA, Date I. [Molecular imaging of transgene expression in brain tumors] Review. No Shinkei Geka. 2004 Aug;32(8):805-24. [Article in Japanese]
Basilion, J.P. Noninvasive monitoring of cellular movements: the utility of MRI and magnetically labeled cells. Invited Inside Blood commentary for Blood, 105: 7-8, January 2005.
Basilion, J.P., Yeon, S., and Bodner, R. “ Magnetic resonance imaging: utility as a molecular imaging modality. ” in: Current Topics in Developmental Biology. Invited chapter, Curr Top Dev Biol. 2005;70:1-33.
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